Describe the theory of language evolution that seems the most plausible to you in your own words. To get a more in-depth understanding of this theory, feel free to read more about it in external sources, but be sure to paraphrase it in your own words and cite the source. Then, discuss why you think this seems the most plausible theory to you? Can you provide some examples from your own observation or understanding of language to support your point?
- Select one poem (must be longer than eight lines) from the WEEK #5 section of the Poetry Reading Schedule.
OPTION 1: Post an image of your handwritten verbatim (word for word) copy of this poem on a standard-sized page of paper (8 ½ x 11) with line breaks and space between stanzas (verse paragraphs). Give the poemâs full title, the poetâs full name, and the poems page number/s in Meyer. Number each verse line (marked by right-hand-marginal line breaks).
OR OPTION 2: Simply type in a verbatim (word for word) copy of this poem on a standard-sized page of paper (8 ½ x 11) with line breaks and space between stanzas (verse paragraphs). Give the poemâs full title, the poetâs full name, and the poems page number/s in Meyer. Number each verse line (marked by right-hand-marginal line breaks).
Sample Solution
cells. For successful cloning, RNA was extracted subsequently cDNA was subjected to amplification using PCR. The plasmids were purified, digested and the amplified cDNA was cloned and transformed into vector. Human leukemic cell line (KCL22) were selected for this study. The Kcl22 cell line is being used extensively owing to its use as exceedingly sensitive target in vitro for various assays. Cultures of ATCC stock exhibit sensitivity towards assessment of human natural killer activity. They are Chronic myelogenous leukemia, BCR-ABL1 positive cell line.These are hematopoietic stem cells having capacity to differentiate to form erythroid, megakaryocytic lineages. The entire RNA content was extracted from KCL22 cell lines, and it was subjected to reverse transcription. The obtained CDNA quality was affirmed by real time qPCR. Amplified product of TROVE2 transcript was cloned into vector subsequent to digestion with selected restriction digestion with pst1 and sgf1. Then, it was ligated by using Bioline QS ligase enzyme. Transformation was carried by heat shock method. Blue white selection strategy was employed to select the transformed colonies. Colony PCR was performed for ascertaining the proper cloning. Agarose gel electrophoresis was employed to separate DNA fragments and analysed fragments cross checked for ascertainment. Results: Tissue culture of KCL22 cells were grown as per routine standard tissue culture conditions. Treated KCL22 cellular samples were grown with media being supplemented with tyrosine kinase inhibitor( TKI) drug for a period of 72h whereas untreated cells were indeed cultured in medium devoid of TKI drug. The morphology of KCL22 cells were subsequently observed by inverted microscope. Viability as well as distribution of size was observed using automated cell counter of BioRad T make. The average percentage has relatively dropped with treated cells (Table 1A and B). Table 1A and B:>
cells. For successful cloning, RNA was extracted subsequently cDNA was subjected to amplification using PCR. The plasmids were purified, digested and the amplified cDNA was cloned and transformed into vector. Human leukemic cell line (KCL22) were selected for this study. The Kcl22 cell line is being used extensively owing to its use as exceedingly sensitive target in vitro for various assays. Cultures of ATCC stock exhibit sensitivity towards assessment of human natural killer activity. They are Chronic myelogenous leukemia, BCR-ABL1 positive cell line.These are hematopoietic stem cells having capacity to differentiate to form erythroid, megakaryocytic lineages. The entire RNA content was extracted from KCL22 cell lines, and it was subjected to reverse transcription. The obtained CDNA quality was affirmed by real time qPCR. Amplified product of TROVE2 transcript was cloned into vector subsequent to digestion with selected restriction digestion with pst1 and sgf1. Then, it was ligated by using Bioline QS ligase enzyme. Transformation was carried by heat shock method. Blue white selection strategy was employed to select the transformed colonies. Colony PCR was performed for ascertaining the proper cloning. Agarose gel electrophoresis was employed to separate DNA fragments and analysed fragments cross checked for ascertainment. Results: Tissue culture of KCL22 cells were grown as per routine standard tissue culture conditions. Treated KCL22 cellular samples were grown with media being supplemented with tyrosine kinase inhibitor( TKI) drug for a period of 72h whereas untreated cells were indeed cultured in medium devoid of TKI drug. The morphology of KCL22 cells were subsequently observed by inverted microscope. Viability as well as distribution of size was observed using automated cell counter of BioRad T make. The average percentage has relatively dropped with treated cells (Table 1A and B). Table 1A and B:>
