Option 1: Why wasn’t the U.S. and its vastly superior intelligence and military able to stop these attacks? How effective are current measures in dealing with attack prevention? Have we really learned from past mistakes?
Option 2: What new problems did the Iraqi War cause for the U.S. and its allies around the world? What effects has it had on the U.S. economy?
Option 3: How much more difficult is it to battle an idea or faith, even a violent one, skewed and brutal than to defeat a nation in war like the U.S. and its allies did during World War II? Consider the role technology plays in the dissemination of faith.
Option 4: Compare European imperialism to current globalism. How has each changed society, both in industrialized nations and developing nations? Is the claim that globalism is a form of imperialism valid?
Option 5: Where do we go from here? Who will the best friends and the worst enemies of the United States be in the coming decades? Will we really, finally achieve that peace and prosperity we all hoped would come to pass?
Sample Solution
Table 1A and B: Preparation of plasmid from E.coli: Preparation of pBluescript SK+ plasmid in E.coli was carried over by growing the E.coli cells with LB media supplemented with ampicillin antibiotic. pBluescript SK+ plasmids were purified using âplasmid purificationâ spin column kit. The concentration of purified plasmid was ascertained using Invitrogen Qubit flourometer by using Quant-IT dsDNA BR dye. Results indicate that the fractions were pure. Table 2: Subsequently, it was subjected to restriction enzyme digestion with restriction digestion buffer. Then treatment with thermo sensitive alkaline phosphatase was carried out to prevent self-ligation. The digestion was ascertained by agarose gel electrophoresis for detection of complete digestion of plasmid (figure 1). Figure 1: The third result belong to student K shows that the different between uncut plasmid and digested plasmid. RNA preparation: Using commercial spin column purification kit , the total RNA was purified from the cells. Then, by using a standard denaturing agarose gel electrophoresis the quality of an RNA sample was ascertained. The RQI value (RNA Quality Indicator) was estimated value of how âintactâ the RNA is in a sample. This value is again being determined by the 28s â 18s ratio of the RNA sample. It will be compared with gel images. Table 3:>
Table 1A and B: Preparation of plasmid from E.coli: Preparation of pBluescript SK+ plasmid in E.coli was carried over by growing the E.coli cells with LB media supplemented with ampicillin antibiotic. pBluescript SK+ plasmids were purified using âplasmid purificationâ spin column kit. The concentration of purified plasmid was ascertained using Invitrogen Qubit flourometer by using Quant-IT dsDNA BR dye. Results indicate that the fractions were pure. Table 2: Subsequently, it was subjected to restriction enzyme digestion with restriction digestion buffer. Then treatment with thermo sensitive alkaline phosphatase was carried out to prevent self-ligation. The digestion was ascertained by agarose gel electrophoresis for detection of complete digestion of plasmid (figure 1). Figure 1: The third result belong to student K shows that the different between uncut plasmid and digested plasmid. RNA preparation: Using commercial spin column purification kit , the total RNA was purified from the cells. Then, by using a standard denaturing agarose gel electrophoresis the quality of an RNA sample was ascertained. The RQI value (RNA Quality Indicator) was estimated value of how âintactâ the RNA is in a sample. This value is again being determined by the 28s â 18s ratio of the RNA sample. It will be compared with gel images. Table 3:>
