Order Description
1: We verified that we purified GFP using western blotting with a specific GFP antibody. What other antibody could be have used to achieve the same conclusion?

2: Could we have used in-gel fluorescence to see if we have purified GFP? Describe what in-gel fluorescence is and what we would have had to do differently to use this technique.

3. How could we use the purified GFP to make a specific antibody against GFP?

– Answer all the questions in ‘detail’
– Diagram to be added to explain the questions
-10 references needed