Q1. During your practical work you cloned the Green Fluorescent Protein (GFP) into the plasmid pGLO and transformed this vector into E. Coli BL21. You induced expression of this protein based on modifications made to the vector that mimicked control of gene expression based on the Arabinose Operon control system. Describe how this system works.

Answer to include:

• Overview of the Arabinose Operon and its regulation. To include control of gene expression by RNA Polymerase, structure of the Promotor, transcription initiation-elongation and termination by RNA Polymerase (10 marks)

• Description of the GFP Promotor and its regulation by ARA-C. Answer to include how this control was based on the Arabinose Operon (10 marks)

• Transformation procedure and GFP induction by Arabinose by inhibiting ARA-C. (10 marks)

• Class results of their transformation experiment(10 marks)