I only question number 2
The bioremediation process shown in the photograph below is used to remove benzene and
other hydrocarbons from soil contaminated by petroleum. The pipes are used to add nitrates, phosphates, oxygen, or water.
Why is each of these ingredients added?
Why is it not always necessary to add bacteria?
3. how does turbidity change as the transmittance decreases?
4. it is unlikely that all your tubes show the same turbidity. How do you explain the variation?
5. why would different members of your group get different transmittance readings for the same temperature? several answers are possible.
6. suppose bacterial cell is placed in a container of suitable size and provided plenty of food in perfect conditions. suppose further that this cell divides asexually at the rate of one division every minutes. How many bacterial cells would be present in 2h? and 4?
7. why does cold temperature influence growth?
8. why does warm temperature influence growth?
9. optimum temperature for growth is not the same in all organism. how can the difference be explained?
10. what turbidity change ( decrease or increase) would you predict over a period of several hours of growth?
11. Turbidity of a given sample must always be completed to that of sterile. Why?
12. the outside of the tubes must be clean before inserting into machine. Why?
13. the broth culture must be agitated before inserting into the colorimeter. Why?
14. special tubes that are optically identical are used to take all reading. Why?
15. What types of food packaging in your home would you expect anaerobic growth to be a problem?
16. you may have dropped hydrogen peroxide on a cut in your skin. What familiar bubbles signify in that that?
17. Why hydrogen peroxide used in this way?
18. Hydrogen peroxide is not considered especially useful to disinfect wounds that contain aerobic bacteria. Why this?
19. why is important to not mix oxygen deep into the tube? how could this have affected your results?
20. you will conclude that your microbe was either aerobic or facultative anaerobic. Why can you safely conclude that your microbe is not obligate anaerobic?
21. Thioglycollate tubes need to be made fresh. What is the risk to accuracy if they sit too long ahead of time before use?
PLEASE SHORT ANSWERS
