BIOLOGY

What    A    Difference    A    Carbon    Makes

T.

K.    Greene
Part III: What a Difference HW
1. Using the data below, construct Lineweaver-Burk plots (double reciprocal) for both reactions (on one

graph) in MS
Excel. (4pts)
I II III
Methanol (mM) Rate of Reaction (mMmin-1
) Rate of Reaction in presence of
5.0 µM Ethanol
0.50 23.5 16.67
1.0 32.2 25.25
1.5 36.9 30.49
2.5 41.8 37.04
3.5 44.0 38.91
2. Calculate the Vmax and the Km for each reaction. How do they compare to your estimated values? (2pts)
3. Do these results support your findings from class? Explain your answer. (2pts)
4. On the basis of the two reactions below, decide which of the following statements are true and which are
false. If a statement is false, explain why it is false. (4pts, 1 each)
1: ATP + Y à Y-P + ADP ?G = –100 kcal/mole
2: Y-P + A à B ?G = 50 kcal/mole
A. Reaction 1 is favorable because of the large negative ?G associated with the hydrolysis of ATP.
B. Reaction 2 is an example of an unfavorable reaction.
C. Reactions 1 and 2 are coupled reactions, and when they take place together, reaction 2 will proceed in the
forward direction.
D. Reaction 2 can be used to drive reaction 1 in the reverse direction.

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