1.Describe the importance of blood vessels network to the body organs and how normal blood vessels are formed? Include to your answer the following key words:• Vaculogenesis- arteriogenesis-angiogenesis• endothelial cells (ECs) from precursors, endothelial cells• Examples of normal physiological angiogenesis e.g embryonic development, adult
2.  What is tumour angiogenesis? Include to your answer the following key words:• Tumour microenvironment• Tumour (Avascular phase, vascular phase)• Tumour angiogenesis mechanisms (sprouting angiogenesis, vessel co-option, intussusception,vasculogenesis, Vasculogenic Mimicry)
3.  Describe tumour Molecular mechanisms of angiogenesis? Include to your answer the following key words:• Introduction• Hypoxia- HIF genes• Angiogenic Switch Mechanism• List of the pro-angiogenic molecules and anti-angiogenic molecules e.g VEGF, VEGF-R,MMP• Proinflammatory cytokines and chemokines
4.   Describe the Physiological Angiogenesis steps in details? Include to your answer the following key words:• Basement degradation, migration and proliferation of endothelial cells … etc

5.   Describe the tumour dysfunctional blood vessels & consequences of the abnormalities on tumour immune response and treatment in details?e.g Oversize diameter and chaotic layout create irregular blood flow .. etc

6.  Describe the role of endothelial cells in immunosuppression in tumour microenvironment in details? Include to your answer the following key words:• Introduction • IMMUNOCHECKPOINT: PD-1 ,PD-L1, PD-L2, CD28, CD80• VEGF, prostaglandin E2 (PGE2), TGF-, IL-6 and IL-10, IL-12
7. Describe the role of Monocytes & Tumour-Associated Macrophages in tumour angiogenesis and immune suppression in the tumour microenvironment in details ? Include to your answer the following key words:• tumour microenvironment • TAM M1 and M2 • Pro-angiogenic and anti-angiogenic molecules secreted by M1 and M2

8. Describe the role of Endothelial cells in leukocytes extravasation to the tumour microenvironment and tumour metastasis?